Dynamic distribution of the avian infectious bronchitis virus isolate strain Jin-13 in SPF chickens / 病毒学报
Chinese Journal of Virology
; (6): 353-358, 2014.
Article
de Zh
| WPRIM
| ID: wpr-280360
Bibliothèque responsable:
WPRO
ABSTRACT
This study aimed to understand the dynamic distribution of infectious bronchitis virus (IBV) Jin-13 strain in SPF chickens. Ninety-day-old SPF chickens were inoculated with Jin-13, a virulent strain, and dissected at day 1, 4, 7, 10, 14, 21, 28 or 35 post-inoculation (dpi). Samples of heart, liver, spleen, lung, trachea, kidney and duodenum were collected and the N gene was detected by Sybr Green I real-time quantitative RT-PCR assays. The established method had a good linear correlation from 7.77 x 10(8) to 10(0) copies/microL. SPF chickens developed typical clinical signs of IBV at the 4th dpi, and the IBV viral concentration of tissues and organs gradually increased with a peak of up to 7.13 x 10(4) copies/microL. The viral concentration of most organs decreased by the 10th dpi, but those of the kidney, trachea and lung remained positive for IBV at 28 dpi and the heart was still positive for IBV at > 35 dpi. The results of this study, showed that the Jin-13 strain can cause prolonged virus excertion in chickens with severe renal damage.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Physiologie
/
Maladies de la volaille
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Trachée
/
Virologie
/
Virulence
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Poulets
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Organismes exempts d'organismes pathogènes spécifiques
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Infections à coronavirus
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Virus de la bronchite infectieuse
/
RT-PCR
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Virology
Année:
2014
Type:
Article