Effect of combination of glutamine and mitomycin C on human gastric carcinoma cell strain MGC-803 / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 345-349, 2006.
Article
de Zh
| WPRIM
| ID: wpr-281201
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of combination of glutamine (GLN) and mitomycin C (MMC) on the human gastric carcinoma cell line MGC-803 in vitro.</p><p><b>METHODS</b>The effects of GLN and MMC were measured by MTT assay, and the interaction between the two agents was evaluated by the median-effect principle. Flow cytometry was used for cell cycle analysis.</p><p><b>RESULTS</b>GLN did not significantly stimulate the cell growth in vitro. High-concentration of GLN could inhibit the cell growth. MMC could effectively inhibit the cell growth in a time-dependent manner. The interaction of these two agents showed a weak antagonistic activity (1 < CI < 1.2703). MMC induced remarkable S-phase arrest. Low-dose GLN has limited effect on the S-phase arrest of MMC, while high-dose GLN significantly attenuated the S-phase arrest and lowered the proliferation index of MGC-803 cell.</p><p><b>CONCLUSIONS</b>Combination of GLN and MMC has a a weak and dose-dependent antagonistic activity in the treatment of gastric carcinoma cell line MGC-803. The combination of high-dose MMC and low-dose GLN may achieve better efficacy.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Pharmacologie
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Tumeurs de l'estomac
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Adénocarcinome
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Mitomycine
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Lignée cellulaire tumorale
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Relation dose-effet des médicaments
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Synergie des médicaments
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Glutamine
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Antibiotiques antinéoplasiques
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2006
Type:
Article