Establishing DNA chip technique for detecting hepatitis C virus genotypes and primary application / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 375-377, 2003.
Article
Dans Zh
| WPRIM
| ID: wpr-281778
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new method based DNA chip technique for detecting HCV genotypes.</p><p><b>METHODS</b>Genotyping probes were designed according to the sequence of HCV 5' NCR to generate DNA chip. The probes on DNA chip contains 5 major genotypes and 8 subtypes. The DNA fragment amplified by labeling Cy5 fluorescence was hybridized with DNA chip.</p><p><b>RESULTS</b>Fifty-five out of 65 isolates detected by DNA chip belonged to 1b- DNA sequencing of form a part of the isolates was used as the control. The results of both were completely consistent.</p><p><b>CONCLUSION</b>The method is simple and rapid with high specificity and sensitivity. It can be applied in detection of HCV RNA and genotypes.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sang
/
ARN viral
/
Réaction de polymérisation en chaîne
/
Sensibilité et spécificité
/
Classification
/
Hepacivirus
/
Régions 5' non traduites
/
Séquençage par oligonucléotides en batterie
/
Génétique
/
Génotype
Type d'étude:
Diagnostic_studies
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2003
Type:
Article