Establishing DNA chip technique for detecting hepatitis C virus genotypes and primary application / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a new method based DNA chip technique for detecting HCV genotypes.</p><p><b>METHODS</b>Genotyping probes were designed according to the sequence of HCV 5' NCR to generate DNA chip. The probes on DNA chip contains 5 major genotypes and 8 subtypes. The DNA fragment amplified by labeling Cy5 fluorescence was hybridized with DNA chip.</p><p><b>RESULTS</b>Fifty-five out of 65 isolates detected by DNA chip belonged to 1b- DNA sequencing of form a part of the isolates was used as the control. The results of both were completely consistent.</p><p><b>CONCLUSION</b>The method is simple and rapid with high specificity and sensitivity. It can be applied in detection of HCV RNA and genotypes.</p>