Inhibition activity of Scutellariae barbata extracts against human hepatocellular carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 591-593, 2006.
Article
de Zh
| WPRIM
| ID: wpr-282973
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibition effect of Scutellariae barbata extracts on the proliferation of human hepatocellular carcinoma cell line QGY-7701.</p><p><b>METHODS</b>The inhibition activity of the extracts against cell line QGY-7701 was estimated by MTT assay. Morphologic changes of the cells were observed under light microscope and electronic microscope. Cell cycle distribution, cell apoptosis and expressions of apoptosis-associated genes as Bcl-2, Bax and Fas were analyzed by flow cytometry.</p><p><b>RESULTS</b>Extracts of Scutellariae barbata could inhibit the proliferation of QGY-7701. When treated with the extracts for 72 h, cells at the early stage of apoptosis showed morphologic changes, apoptotic cells increased, cells at G0/G1 phase decreased and those at G2/M phase increased, the expression of Bax significantly up-regulated and that of Bcl-2 down-regulated with no change in Fas gene.</p><p><b>CONCLUSION</b>The extracts of Scutellariae barbata inhibit the proliferation of QGY-7701, which may relate to the activation of anti-oncogene Bcl-2, induction of cell apoptosis and inhibition of G to S phase cell cycle progress.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Pharmacologie
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Médicaments issus de plantes chinoises
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Extraits de plantes
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Cycle cellulaire
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Apoptose
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Carcinome hépatocellulaire
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Antigènes CD95
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Protéines proto-oncogènes c-bcl-2
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Scutellaria baicalensis
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2006
Type:
Article