Cloning, expression and immunocharacterization of the capsid protein of human Norwalk virus Guangzhou strain NVgz01 / 南方医科大学学报
Journal of Southern Medical University
; (12): 1410-1413, 2007.
Article
Dans Zh
| WPRIM
| ID: wpr-283118
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To clone, express and characterize the capsid protein of human Norwalk virus Guangzhou strain NVgz01.</p><p><b>METHODS</b>On the basis of successful construction of full-genome clones and sequence analysis of human norovirus Guangzhou strain NVgz01, the full capsid gene was ligated into pET28a (+) for expression. After IPTG induction, the recombinant protein was purified through metal (Ni(2+)) chelating affinity chromatography. Western blotting and enzyme-linked immunosorbent assay (ELISA) were used to determine the antigenicity of the recombinant protein.</p><p><b>RESULTS</b>The recombinant capsid gene was overexpressed in E.coli, yielding the recombinant protein with relative molecular mass of 62x10(3) that was highly purified through metal (Ni(2+)) chelating affinity chromatography. IDEIA Norovirus Kit and immunoassay showed that the recombinant protein had good antigenicity.</p><p><b>CONCLUSION</b>The capsid gene of norovirus Guangzhou strain has been cloned and expressed, which can be useful for developing diagnostic reagents or vaccine of norovirus.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Plasmides
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Test ELISA
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Expression des gènes
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Technique de Western
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Clonage moléculaire
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Virus de Norwalk
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Protéines de capside
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Allergie et immunologie
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Génétique
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2007
Type:
Article