The hyposensitization of oval cell to antiproliferative effect of transforming growth factor β1 by hepatitis B virus X gene / 中华外科杂志
Chinese Journal of Surgery
; (12): 432-435, 2011.
Article
Dans Zh
| WPRIM
| ID: wpr-285707
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether hepatitis B virus X (HBX) protein expression affect the oval cells' response to anti-proliferative effect of transforming growth factor β1 (TGFβ1) in oval cells.</p><p><b>METHODS</b>Real-time PCR, Western blot analysis were performed to detect the expression of TGFβRII in HBX-transfected oval cells named HBX-EGFP-LE/6, and EGFP-LE/6, LE/6 control cells. In addition, exogenous TGFβ1 was added into all three oval cell lines, MTT assay was preformed to clarify different responses to the anti-proliferative effect of TGFβ1.</p><p><b>RESULTS</b>The TGFβRII mRNA levels in LE/6 and EGFP-LE/6 cells were (10.2 ± 1.8) and (8.8 ± 0.9) folds of those in HBX-EGFP-LE/6 cells, the difference was significant (P < 0.05). HBX protein expression also reduced the protein levels of TGFβRII in HBX-EGFP-LE/6 oval cells, compared to the control cells. The MTT results exhibited that, after TGFβ1 addition, proliferative inhibition rate in the HBX-EGFP-LE/6 cells was 18.1% ± 1.5% while those in control cells were 42.2% ± 2.8% and 41.9% ± 5.0%, the difference was significant (P < 0.01).</p><p><b>CONCLUSION</b>HBX protein expression affects TGFβRII transcriptional activity and protein synthesis, and insensitive oval cells to anti-proliferative effect of TGFβ1.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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ARN messager
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Transfection
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Transactivateurs
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Lignée cellulaire
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Biologie cellulaire
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Prolifération cellulaire
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Facteur de croissance transformant bêta-1
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Génétique
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Foie
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Surgery
Année:
2011
Type:
Article