Histological features of tumor-bearing tissues formed by human fibroblasts after reprograming by Piwil2 / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 565-572, 2015.
Article
Dans Zh
| WPRIM
| ID: wpr-286779
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the histological features of tumor-bearing tissues formed by human fibroblasts after reprograming by spermatogonial stem cell self-renewal key regulating gene Piwil2 (Piwil2-iCSC).</p><p><b>METHODS</b>Piwil2-iCSC tumor spheroids-like colonies were selected for tumor formation assay in four nude mice. Pathological features of Piwil2-iCSC tumors were observed by histology. Stem cell markers and common triploblastic markers were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) assay and immunohistochemistry. Germ cell tumor markers were detected by immunohistochemical examination.</p><p><b>RESULTS</b>Two weeks after inoculation, subcutaneous tumors were formed in all the four nude mice with a tumor formation rate of 100%. In the Piwil2-iCSC tumor tissues, Piwil2-GFP(+) cells showed high-density nuclear expression and were widely observed in DAPI-stained sections. Numerous mitotic figure of the neoplastic cells were seen (>10 cells/field of vision under high magnification) in HE-stained sections. Enlarged abnormal cell nuclei were observed. RT-PCR assay showed that Piwil2-iCSC tumors still expressed Piwil2 and some self-renewal and pluripotent markers of stem cells and some markers of triploblastic differentiation. Immunohistochemical staining showed that the tumors expressed stem cell markers, triploblastic markers and germ cell tumor markers AFP and HCG.</p><p><b>CONCLUSIONS</b>Piwil2-iCSC tumors are probably undifferentiated embryonic small cell carcinoma, most likely to be immature teratoma, mixed with yolk sac tumor and choriocarcinoma components. It can be used as a useful model for the research of origin or genesis mechanism of cancer stem cells and the treatment of relevant tumors.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Tératome
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Facteurs temps
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Cellules souches tumorales
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Immunohistochimie
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Choriocarcinome
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Chimie
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Tumeur du sac vitellin
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Tumeurs embryonnaires et germinales
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Sphéroïdes de cellules
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
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Humans
langue:
Zh
Texte intégral:
Chinese Journal of Oncology
Année:
2015
Type:
Article