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Expression of imprinted genes during the course of differentiation from mouse embryonic stem cells to islet-like cells in vitro / 中国当代儿科杂志
Zhongguo dangdai erke zazhi ; Zhongguo dangdai erke zazhi;(12): 954-958, 2010.
Article de Zh | WPRIM | ID: wpr-286937
Bibliothèque responsable: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of in vitro inducement on the expression of SF1-G imprinted genes, Kcnq1 and Cdkn1c during the course of differentiation from mouse embryonic stem (ES) cells to islet-like cells.</p><p><b>METHODS</b>Mouse ES cells were induced to differentiate into islet-like cells in vitro. The expression of islet specific markers was tested by RT-PCR or immunofluorescence. RT-PCR/RFLP was used to test the imprinted genes parental expression in cells at different stages.</p><p><b>RESULTS</b>Islet specific genes, such as Insulin, Glucagon, Somatostatin, IAPP and Glut2, were expressed in differentiated cells. The proteins of insulin, C-peptide and Somastatin were expressed in the final stage cells. Imprinted gene Kcnq1 and Cdkn1c were biallelicly expressed in islet-like cells.</p><p><b>CONCLUSIONS</b>Mouse ES cells can be successfully induced into islet-like cells in vitro. Gene imprinting status of Kcnq1 and Cdkn1c may be changed in differentiated cells (causing loss of imprinting) during the in vitro inducement.</p>
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Cellules souches / Protéines / Différenciation cellulaire / Ilots pancréatiques / RT-PCR / Biologie cellulaire / Cellules souches embryonnaires de souris / Insuline Limites du sujet: Animals langue: Zh Texte intégral: Zhongguo dangdai erke zazhi Année: 2010 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Cellules souches / Protéines / Différenciation cellulaire / Ilots pancréatiques / RT-PCR / Biologie cellulaire / Cellules souches embryonnaires de souris / Insuline Limites du sujet: Animals langue: Zh Texte intégral: Zhongguo dangdai erke zazhi Année: 2010 Type: Article