Primary evaluation of anti-HEV diagnostic reagent by experimental infection animal model with hepatitis E virus / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 48-51, 2008.
Article
de Zh
| WPRIM
| ID: wpr-287821
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate anti-HEV diagnostic kits by experimental infecting rhesus monkeys with HEV.</p><p><b>METHODS</b>Eight rhesus monkeys were infected with genotype 1 and 4 HEV separately. The alanine aminotransferase (ALT) level of all monkeys were detected before and after the process of infection. HEV RNA in stool specimens was tested by reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Anti-HEV IgG in serum was detected by GL-IgG and WT-IgG.</p><p><b>RESULTS</b>HEV RNA presented in the stool of all the 8 monkeys after infection. The ALT level of 1 monkey infected with genotype 1 HEV and 2 monkeys infected with genotype 4 HEV appeared abnormally after infection. Tested by GL-IgG, 2 of the 4 monkeys infected with genotype 1 HEV and 1 of 4 monkeys infected with genotype 4 HEV seroconverted to anti-HEV IgG. However, when tested by WT-IgG, all the infected monkeys seroconverted to anti-HEV IgG. The anti-HEV IgG tested by WT-IgG was positive during the whole observation period,and the anti-HEV IgG measured by GL-IgG only remained 12 weeks after infection. Detected by GL-IgG and WT-IgG, seropositive conversion of the anti-HEV IgG happened almost at the same time.</p><p><b>CONCLUSION</b>Both GL-IgG and WT-IgG could detect the anti-HEV IgG of experimentally infected rhesus monkeys but the WT-IgG had a higher sensitivity for detection of anti-HEV IgG than</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Virologie
/
Sang
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Immunoglobuline G
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Virus de l'hépatite E
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Hépatite E
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RT-PCR
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Alanine transaminase
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Modèles animaux de maladie humaine
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Allergie et immunologie
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Génétique
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Epidemiology
Année:
2008
Type:
Article