Establishment of serum peptidome pattern-based diagnostic model of endometriosis / 南方医科大学学报
Journal of Southern Medical University
; (12): 851-854, 2010.
Article
de Zh
| WPRIM
| ID: wpr-290044
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a diagnostic model of endometriosis by analyzing serum peptidome patterns in patients with endometriosis using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS).</p><p><b>METHODS</b>Serum samples from 21 endometriosis patients and 29 healthy control subjects were analyzed using MALDI-TOF-MS and Clinprotools 2.0 software to establish the diagnostic model of endometriosis, which was validated using the serum samples from 10 endometriosis patients and 15 healthy controls.</p><p><b>RESULTS</b>Eighteen statistically significant peptide peaks were obtained with the m/z value ranging from 1,000 to 10,000 (P<0.01). Among the peaks, 12 were down-regulated and 6 up-regulated. The sensitivity and specificity of the model was 90.9% and 100.0%, respectively, with a diagnostic accuracy of 96.2%.</p><p><b>CONCLUSION</b>This model shows the feasibility of using MALDI-TOF-MS and Clinprotools software to identify the biomarkers of endometriosis.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sang
/
Marqueurs biologiques
/
Études de faisabilité
/
Sensibilité et spécificité
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Spectrométrie de masse MALDI
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Protéome
/
Protéomique
/
Diagnostic
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Endométriose
/
Méthodes
Type d'étude:
Diagnostic_studies
Limites du sujet:
Adult
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Female
/
Humans
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2010
Type:
Article