Subtype and sequence analysis of human immunodeficiency virus type-1 strains in Guangdong province / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 344-347, 2006.
Article
de Zh
| WPRIM
| ID: wpr-290261
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate subtype and genetic analysis of human immunodeficiency virus type-1 (HIV-1).</p><p><b>METHODS</b>DNA sequences were amplified by nested-PCR from uncultured peripheral blood mononuclear cells (PBMC) obtained from 100 HIV-1 patients from Guangdong Province. The C2 to V3 region of the envelope glycoprotein gp120 of HIV-1 was sequenced directly. The analysis of the gene-based phylogenetic tree and variation of amino acid were carried out by using Wisconsin software package or genetics computer group (GCG).</p><p><b>RESULTS</b>DNA fragments were amplified from 75 PBMC samples by using nested polymerase chain reaction (PCR). Sequence analysis showed that there were 3 HIV-1 subtypes or circulating recombinant forms (CRF): CRF01-AE (n = 44), CRF-BC (n = 27) and B' (n = 4).</p><p><b>CONCLUSIONS</b>Three HIV-1 subtypes or circulating recombinant forms: CRF01-AE, CRF-BC and B' might be circulating in Guangdong Province. Findings from this study suggested that several subtypes might exist in Guangdong Province and the epidemic situation of AIDS be serious. It should be a challenge for Guangdong Province in treating patients, preventing and controlling AIDS in the future.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Virologie
/
Sang
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ADN viral
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Séquence nucléotidique
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Chine
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Épidémiologie
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Syndrome d'immunodéficience acquise
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VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
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Classification
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Isoformes de protéines
Limites du sujet:
Adolescent
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Adult
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Child
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Female
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Humans
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Male
Pays comme sujet:
Asia
langue:
Zh
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2006
Type:
Article