Determination of three constituents in Radix Astragali by HPLC-MS / 药学学报
Acta Pharmaceutica Sinica
; (12): 793-796, 2006.
Article
de Zh
| WPRIM
| ID: wpr-294937
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To determine calycosin-7-O-beta-D-glucoside, astragaloside IV and formononetin in Radix Astragali and other relative samples by HPLC-MS.</p><p><b>METHODS</b>HPLC was carried out with Agilent 1100LC/MSD, equipped with Agilent Zorbax SB C18 column (250 mm x 4.6 mm ID, 5 microm) and mass spectrum detector. The mobile phase (CH3CN-H2O) was eluted in gradient mode.</p><p><b>RESULTS</b>The calibration curves of calycosin-7-O-beta-D-glucoside, astragaloside IV and formononetin were linear in the range of 0.03 - 1.21 microg x mL(-1), 0.35 - 13.86 microg x mL(-1) and 0.38 - 15.22 microg x mL(-1), respectively. These recoveries of samples were from 95% to 105% with RSD less than 1.5%.</p><p><b>CONCLUSION</b>The method was employed to analyse 25 samples of Radix Astragali and other relative samples, including Radix Astragali slice, Radix Astragali Preparata, Hedysarum polybotrys Hand. -Mazz, Astragalus ernestii Comb. The contents of three constituents vary greatly because of the species, place of collection and season of harvesting. This method could apply to evaluate the quality of Radix Astragali and it is simple, sensitive and reliable.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Plantes médicinales
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Saponines
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Saisons
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Spécificité d'espèce
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Triterpènes
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Chimie
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Chine
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Reproductibilité des résultats
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Chromatographie en phase liquide à haute performance
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Écosystème
Pays comme sujet:
Asia
langue:
Zh
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2006
Type:
Article