Beneficial effects of human umbilical cord blood mononuclear cells transplantation on rats with experimental myocardial infarction / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 587-590, 2006.
Article
Dans Zh
| WPRIM
| ID: wpr-295273
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Human umbilical cord blood contains abundant immature stem/progenitor cells, which may contribute to the repair of infarcted myocardium. Present study aimed to explore the feasibility and effects of human umbilical cord blood mononuclear cells (HUCBC) transplantation for the treatment of myocardial infarction.</p><p><b>METHODS</b>Forty-five male Wistar rats were randomly divided into three groups (1) Myocardial infarction (MI plus vehicle, n = 15), (2) MI plus cell transplantation (HUCBC were implanted into the peri-infarct area immediately after MI, n = 15), (3) Normal control group (n = 15). After echocardiography and hemodynamic measurements, the rats were sacrificed for histological and immunochemical examinations one month post MI.</p><p><b>RESULTS</b>The transplanted HUCBC survived and participated the repair process in host heart. Significantly improved left ventricular function was evidenced by echocardiography in cell transplantation group compared to the MI control group. Left ventricular end-diastolic pressure was significantly reduced LVEDP (21.08 +/- 8.10) vs (30.82 +/- 9.59) mm Hg, P < 0.05], +dp/dt(max) [(4.29 +/- 1.27) vs (3.24 +/- 0.75) mm Hg/ms, P < 0.05] and -dp/dt(max) increased [(3.71 +/- 0.79) vs (3.00 +/- 0.49) mm Hg/ms, P < 0.05] in cell transplantation rats compared with MI control rats. vWF immunostaining examination showed significantly increased microvessels within the boundary of infarcted myocardium in cell transplantation group compared to the MI control group (P < 0.01).</p><p><b>CONCLUSIONS</b>HUCBC transplantation may improve cardiac function in MI rats by promoting microvessel formation.</p>
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Chirurgie générale
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Répartition aléatoire
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Rat Wistar
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Transplantation de cellules souches de sang du cordon
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Infarctus du myocarde
Type d'étude:
Clinical_trials
Limites du sujet:
Animals
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Humans
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Male
langue:
Zh
Texte intégral:
Chinese Journal of Cardiology
Année:
2006
Type:
Article