Construction, expression and immunogenicity analysis of a fusion protein containing M2e of influenza A virus fused to a modified Pseudomonas aeruginosa exotoxin A / 病毒学报
Chinese Journal of Virology
; (6): 189-194, 2010.
Article
de Zh
| WPRIM
| ID: wpr-297885
Bibliothèque responsable:
WPRO
ABSTRACT
M2 protein of type A influenza virus is a good candidate for universal influenza vaccine, exotoxin A of Pseudomonas aeruginosa may facilitate the immunogenicity of M2 protein. We constructed and expressed a prokaryotic expression plasmid containing a chimeric gene of M2 extracellular coding region and a partial PEA gene, and observed the immunoprotection in BALB/c mice vaccinated with the fusion protein. The fusion protein (ntPE-M2e) was generated by inserting the coding sequence of the M2e in place of Ib loop in PEA. This fusion protein was used to immunize BALB/c mice by subcutaneously injection with incomplete Freund's adjuvant and boost at weeks 3 and 7. The immunized mice were challenged with influenza virus strain A/PR/34/8. The fusion protein (ntPE-M2e) immunization protected mice against lethal viral challenge. ELISA and ELISPOT results demonstrated that the fusion protein could induce a strong systemic immune response against synthetic M2e peptide, and virus replication in the lungs of mice was inhibited in comparison with the control. This study provides foundation for developing broad-spectrum vaccines against type A influenza viruses.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Virus de la grippe A
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Physiologie
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Toxines bactériennes
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Virologie
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Protéines de fusion recombinantes
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Test ELISA
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Expression des gènes
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Protéines de la matrice virale
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Immunisation
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ADP ribose transferases
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Virology
Année:
2010
Type:
Article