Antibody preparation and enzyme linked immunosorbent assay of Lonicera japonica phenylalnine ammonia lyase / 药学学报
Acta Pharmaceutica Sinica
; (12): 1498-1502, 2013.
Article
Dans Zh
| WPRIM
| ID: wpr-298052
Responsable en Bibliothèque :
WPRO
ABSTRACT
The expression of phenylalnine ammonia lyase (LJPAL1) is closely related to the content of active compounds in Lonicera japonica. In this paper, a prokaryotic expression vector is constructed and LJPAL1 protein is expressed in E. coli. Three antigen sites were synthesized to peptide antigen and prepared polyclonal antibody of Anti-LJT-1, Anti-LJT-2 and Anti-LJT-3, separately. Antibody Anti-LJT-2 was screened using Western blotting. And indirect ELISA was built using Anti-LJT-2. The results of this study will be a base for honeysuckle chemical quality and evaluation kits.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phenylalanine ammonia-lyase
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Plantes médicinales
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Plasmides
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Test ELISA
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Chimie
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Technique de Western
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Structure tertiaire des protéines
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Structure secondaire des protéines
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Régulation de l'expression des gènes végétaux
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Lonicera
langue:
Zh
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2013
Type:
Article