Synthesis and identification of artificial antigens of paneoniflorin / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2043-2046, 2014.
Article
de Zh
| WPRIM
| ID: wpr-299834
Bibliothèque responsable:
WPRO
ABSTRACT
Oxidation method with sodium iodide was used to synthesize immunogenic antigen (PF-BSA) and coating antigen (PF-OVA) of paeoniflorin. UV spectroscopy showed that paeoniflorin was successfully conjugated with BSA and OVA. After immunized by PF-BSA, the mice can produce anti-paeoniflorin antibodies specifically. The ELISA test results showed the high titer (1:12 800) and specificity (IC50 = 0.791 mg x L(-1)) of the antiserum from mice injected with PF-BSA. Also, the antiserum showed low cross activities against nine traditional Chinese medicine (TCM) of small molecules. These artificial antigens were successfully synthesized and the anti-paeoniflorin antibody well prepared, which provides the experimental basis for the further study of ELISA and its kit.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sérumalbumine bovine
/
Test ELISA
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Médicaments issus de plantes chinoises
/
Chimie
/
Monoterpènes
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Allergie et immunologie
/
Glucosides
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Souris de lignée BALB C
/
Anticorps
/
Antigènes
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2014
Type:
Article