Effect of Antrodia cinnamomea on gene expression related to aortal endothelial injury in rats with hyperlipidemia / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1670-1674, 2014.
Article
Dans Zh
| WPRIM
| ID: wpr-300208
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Antrodia cinnamomea on gene expression related to aortal endothelial injury of rats with hyperlipidemia.</p><p><b>METHOD</b>Fifty SD rats were randomly divided into five groups the normal control group (NG), the model group (MG), the antrodia cinnamomea groups of low, middle and high doses (AC-LG, AC-MG, AC-HG, 250, 500, 1 000 mg x kg(-1)). The rats were fed with high-fat diets to establish the hyperlipidemia model. After the drug administration for 10 weeks, their serum lipid, SOD, MDA and ox-LDL, LOX-1, P38 MAPK and NF-kappaB mRNA and protein expression were respectively determined, and the aortal endothelial injury was observed under electron microscope.</p><p><b>RESULT</b>In the model group, the contents of TC, TG and LDL-C significant increased (P < 0.01), whereas the content of HDL-C significant decreased (P < 0.01). Compared with the model group, both the AC-M group and the AC-H group showed reduction in endothelial injury and significant decrease in the content of TC, TG and LDL-C (P < 0.05 or P < 0.01). The content of HDL-C increased, but with no significant difference. SOD activity in serum remarkably increased (P < 0.05 or P < 0.01), MDA and ox-LDL levels dramatically decreased (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>A. cinnamomea can alleviate endothelial lipid injury by inhibiting the expressions of LOX-1, P38MAPK and NF-kappaB in aorta and better protect aortal endothelial cells from oxidative lipid injury.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Aorte
/
Anatomopathologie
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Pharmacologie
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Superoxide dismutase
/
Triglycéride
/
Produits biologiques
/
Sang
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Test ELISA
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Endothélium vasculaire
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Microscopie électronique
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2014
Type:
Article