Multiplex real-time PCR method for rapid detection of Marburg virus and Ebola virus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 313-315, 2012.
Article
Dans Zh
| WPRIM
| ID: wpr-305048
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Marburg virus and Ebola virus are acute infections with high case fatality rates. A rapid, sensitive detection method was established to detect Marburg virus and Ebola virus by multiplex real-time fluorescence quantitative PCR.</p><p><b>METHODS</b>Designing primers and Taqman probes from highly conserved sequences of Marburg virus and Ebola virus through whole genome sequences alignment, Taqman probes labeled by FAM and Texas Red, the sensitivity of the multiplex real-time quantitative PCR assay was optimized by evaluating the different concentrations of primers and Probes.</p><p><b>RESULTS</b>We have developed a real-time PCR method with the sensitivity of 30.5 copies/microl for Marburg virus positive plasmid and 28.6 copies/microl for Ebola virus positive plasmids, Japanese encephalitis virus, Yellow fever virus, Dengue virus were using to examine the specificity.</p><p><b>CONCLUSIONS</b>The Multiplex real-time PCR assays provide a sensitive, reliable and efficient method to detect Marburg virus and Ebola virus simultaneously.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Virologie
/
Infections à Filoviridae
/
Fièvre hémorragique à virus Ebola
/
Diagnostic
/
Ebolavirus
/
Réaction de polymérisation en chaine multiplex
/
Réaction de polymérisation en chaine en temps réel
/
Génétique
/
Marburgvirus
/
Méthodes
Type d'étude:
Diagnostic_studies
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2012
Type:
Article