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Screening of differential proteins binding to Nox1 promoter in A549 cell model of inflammation and oxidative stress / 南方医科大学学报
Article de Zh | WPRIM | ID: wpr-306484
Bibliothèque responsable: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen the regulatory proteins involved in Nox1 promoter activation in a cell model of inflammation and oxidative stress.</p><p><b>METHODS</b>A cell model of inflammation and oxidative stress was established by stimulating A549 cells with tumor necrosis factor-α (TNF-α). The differential proteins binding to Nox1 promoter were screened by DNA pull-down and the binding proteins were separated by 2D electrophoresis and selected according to the their differential expression levels (with over 1.5-fold changes relative to the control level). The screened proteins were finally identified by MALDI-TOF/TOF-MS.</p><p><b>RESULTS</b>Seven differentially expressed protein spots (all upregulated in the cell model) were obtained, among which GLE1, DDX19A, KRT1 and KRT10 were identified by mass spectrometry.</p><p><b>CONCLUSION</b>GLE1, DDX19A, KRT1 and KRT10 participate in the activation of Nox1 promoter in TNF-α-induced A549 cells, and this result provides new insights into the biological roles of the regulatory proteins of Nox1 promoter in inflammation and oxidative stress.</p>
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Spectrométrie de masse / Électrophorèse bidimensionnelle sur gel / Régions promotrices (génétique) / Facteur de nécrose tumorale alpha / Stress oxydatif / NADPH oxidase / Transporteurs nucléocytoplasmiques / Lignée cellulaire tumorale / Kératine-10 / Kératine-1 Type d'étude: Diagnostic_studies / Prognostic_studies / Screening_studies Limites du sujet: Humans langue: Zh Texte intégral: Journal of Southern Medical University Année: 2013 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Spectrométrie de masse / Électrophorèse bidimensionnelle sur gel / Régions promotrices (génétique) / Facteur de nécrose tumorale alpha / Stress oxydatif / NADPH oxidase / Transporteurs nucléocytoplasmiques / Lignée cellulaire tumorale / Kératine-10 / Kératine-1 Type d'étude: Diagnostic_studies / Prognostic_studies / Screening_studies Limites du sujet: Humans langue: Zh Texte intégral: Journal of Southern Medical University Année: 2013 Type: Article