Screening of differential proteins binding to Nox1 promoter in A549 cell model of inflammation and oxidative stress / 南方医科大学学报
Journal of Southern Medical University
; (12): 703-707, 2013.
Article
de Zh
| WPRIM
| ID: wpr-306484
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen the regulatory proteins involved in Nox1 promoter activation in a cell model of inflammation and oxidative stress.</p><p><b>METHODS</b>A cell model of inflammation and oxidative stress was established by stimulating A549 cells with tumor necrosis factor-α (TNF-α). The differential proteins binding to Nox1 promoter were screened by DNA pull-down and the binding proteins were separated by 2D electrophoresis and selected according to the their differential expression levels (with over 1.5-fold changes relative to the control level). The screened proteins were finally identified by MALDI-TOF/TOF-MS.</p><p><b>RESULTS</b>Seven differentially expressed protein spots (all upregulated in the cell model) were obtained, among which GLE1, DDX19A, KRT1 and KRT10 were identified by mass spectrometry.</p><p><b>CONCLUSION</b>GLE1, DDX19A, KRT1 and KRT10 participate in the activation of Nox1 promoter in TNF-α-induced A549 cells, and this result provides new insights into the biological roles of the regulatory proteins of Nox1 promoter in inflammation and oxidative stress.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Spectrométrie de masse
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Électrophorèse bidimensionnelle sur gel
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Régions promotrices (génétique)
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Facteur de nécrose tumorale alpha
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Stress oxydatif
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NADPH oxidase
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Transporteurs nucléocytoplasmiques
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Lignée cellulaire tumorale
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Kératine-10
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Kératine-1
Type d'étude:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2013
Type:
Article