In vitro effect of zilongjin on prostate cancer cell line LNCaP / 中国中西医结合杂志
Zhongguo Zhong Xi Yi Jie He Za Zhi
; (12): 621-624, 2004.
Article
de Zh
| WPRIM
| ID: wpr-306830
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Zilongjin (ZLJ) on human androgen-dependent type of prostate cancer cell line LNCaP.</p><p><b>METHODS</b>MTT assay, flow cytometry and fluorescence microscopy were used to observe the effect of ZLJ in anti-proliferation, cell cycle arresting and apoptosis induction. RT-PCR was used to examine the effect of ZLJ on expressions of prostate marker gene (PSA), androgen receptor (AR), apoptosis related genes (bcl-2 and bax), and Western blot assay was used to detect the effect on protein expression of bcl-2 and bax.</p><p><b>RESULTS</b>ZLJ could cause apparent inhibition on proliferation, induce G0/G1 phase arresting and apoptosis in time- and dose-dependent manner on LNCaP cells. The concentration for inhibiting cell growth by 50% (IC50) in 72 hrs was 0.79 mg/ml. ZLJ could down-regulate the expression of PSA, AR, bcl-2 genes and lower bcl-2 protein expression, but showed ineffective on bax protein expression.</p><p><b>CONCLUSION</b>ZLJ displays its anti-tumor effects by way of inhibiting the cell proliferation, arresting the G0/G1 phase, inducing apoptosis, down-regulating PSA, AR, bcl-2 gene expression and lowering bcl-2 protein expressions.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Pharmacologie
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Tumeurs de la prostate
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Médicaments issus de plantes chinoises
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Récepteurs aux androgènes
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Antigène spécifique de la prostate
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Protéines proto-oncogènes c-bcl-2
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Lignée cellulaire tumorale
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Prolifération cellulaire
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Génétique
Limites du sujet:
Humans
/
Male
langue:
Zh
Texte intégral:
Zhongguo Zhong Xi Yi Jie He Za Zhi
Année:
2004
Type:
Article