Inverse correlation between Snail and E-cadherin expression in carcinoma cell lines and invasive ability in vitro / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 17-20, 2006.
Article
de Zh
| WPRIM
| ID: wpr-308432
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Malignant transformation of epithelial cell frequently coincides with loss of E-cadherin. Here we study the expression of Snail and E-cadherin and correlate their expression with cell differentiation and in vitro invasion.</p><p><b>METHODS</b>The expression and localization of Snail and E-cadherin were studied by Northern blot and laser confocal microscopy in two normal cell lines (MDCK, NIH 3T3) and six carcinoma cell lines (A431, MCF-7, MDA-MB-453, HepG2, MDA-MB-435s, MDA-MB-231). Boyden chamber assay was done to detect the invasive ability of cells in vitro.</p><p><b>RESULTS</b>Snail mRNA and protein were detected in fibroblasts NIH 3T3 and poorly differentiated carcinoma cell lines HepG2, MDA-MB-435s and MDA-MB-231. On the contrary, E-cadherin mRNA and protein were detected in normal epithelial cell line MDCK and well differentiated carcinoma cell lines A431 and MDA-MB-453. In MCF-7 cells, Snail and E-cadherin expressions were revealed both at mRNA and protein levels. The cells with higher expression of Snail had stronger ability of invasion than those with lower expression of Snail.</p><p><b>CONCLUSION</b>There is an inverse correlation between Snail and E-cadherin expressions and their expressions are correlated with cell differentiation and tumor invasiveness.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Facteurs de transcription
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Cadhérines
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Différenciation cellulaire
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Lignée cellulaire
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Transformation cellulaire néoplasique
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Cellules 3T3
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Biologie cellulaire
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Lignée cellulaire tumorale
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Cellules épithéliales
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Facteurs de transcription de la famille Snail
Limites du sujet:
Animals
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Humans
langue:
Zh
Texte intégral:
Chinese Journal of Oncology
Année:
2006
Type:
Article