Regularity of changes in chlorogenic acids in Lonicera japonica extracts by HPLC-DAD-ESI-MS/MS / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3564-3568, 2012.
Article
Dans Zh
| WPRIM
| ID: wpr-308577
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze chlorogenic acids contained in Lonicera japonica water extracts, and to investigate the regularity of changes in chlorogenic acids during the water extraction process.</p><p><b>METHOD</b>Agilent Zorbax SB-C18 analytical column (4.6 mm x 250 mm, 5 microm) was eluted with 0.1% formic acid (A)-acetonitrile (B) as the mobile phase at a flow rate of 0.5 mL x min(-1). The detection wavelength was 327 nm and the column temperature was 30 degrees C. Negative MS(n) mode was adopted in mass spectrum for ananlyzing the 22 samples of L. japonica water extracts.</p><p><b>RESULT</b>Caffeic acid and six organic acids were accurately identified from the water extracts. During the extraction, the contents of chlorogenic acid and 3,5-dicaffeoylquinic acid became stable or gradually decreased after reaching the highest value. The contents of other components had long been increasing, but with a decreasing rate of change.</p><p><b>CONCLUSION</b>This study provides basis for improving the production process of traditional Chinese medicine preparations containing L. japonica.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Médicaments issus de plantes chinoises
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Structure moléculaire
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Chimie
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Acide chlorogénique
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Chromatographie en phase liquide à haute performance
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Spectrométrie de masse ESI
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Lonicera
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Spectrométrie de masse en tandem
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Méthodes
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2012
Type:
Article