Insertion of TC motif into hepatitis B virus core protein c/e1 site does not affect the expression of S and e antigen / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 585-588, 2009.
Article
de Zh
| WPRIM
| ID: wpr-310041
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether insertion of TC motif into hepatitis B virus (HBV) core protein c/e1 site affects the expression of S and e antigen.</p><p><b>METHODS</b>Different oligonucleotides encoding TC motif were inserted into the c/e1 site of the core gene of a 1.3 copy wild-type HBV genome vector. HepG2 cells were divided into several groups of cells to transiently transfect with the wild-type and mutant HBV vectors, respectively. In each group, the expression level of core protein inside cells was detected by western blotting, and the levels of S and e antigen in culture medium were analyzed by ELISA assay.</p><p><b>RESULTS</b>Western blotting showed that these TC-tagged core proteins were expressed at similar level of wild-type one. ELISA assay indicated that the level of S and e antigen in culture medium of different groups were not significantly different.</p><p><b>CONCLUSION</b>Insertion of TC motif into HBV core protein c/e1 site does not interference with the expression of viral protein encoded by HBV genome.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protéines virales
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Réplication virale
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Protéines recombinantes
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Test ELISA
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Transfection
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Protéines du core viral
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Virus de l'hépatite B
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Technique de Western
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Mutagenèse par insertion
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Séquence d'acides aminés
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Hepatology
Année:
2009
Type:
Article