Differential Proteomics Reveals the Potential Injury Mechanism Induced by Heavy Ion Radiation in Mice Ovaries / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 301-307, 2017.
Article
de En
| WPRIM
| ID: wpr-311410
Bibliothèque responsable:
WPRO
ABSTRACT
In the present study, we used a proteomics approach based on a two-dimensional electrophoresis (2-DE) reference map to investigate protein expression in the ovarian tissues of pubertal Swiss-Webster mice subjected to carbon ion radiation (CIR). Among the identified proteins, ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) is associated with the cell cycle[1] and that it influences proliferation in ovarian tissues. We analyzed the expression of UCH-L1 and the proliferation marker proliferation cell nuclear antigen (PCNA) following CIR using immunoblotting and immunofluorescence. The proteomics and biochemical results provide insight into the underlying mechanisms of CIR toxicity in ovarian tissues.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Ovaire
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Effets des rayonnements
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Marqueurs biologiques
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Électrophorèse bidimensionnelle sur gel
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Protéines de transport
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Répartition aléatoire
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Expression des gènes
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Protéomique
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Ubiquitin thiolesterase
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Radiothérapie par ions lourds
Limites du sujet:
Animals
langue:
En
Texte intégral:
Biomedical and Environmental Sciences
Année:
2017
Type:
Article