Reverse genetics platform construction of influenza pandemic virus strain / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 85-88, 2013.
Article
de Zh
| WPRIM
| ID: wpr-318097
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Reverse genetics was used to construct the platform of flu pandemic strain A/California/07/2009 (H1N1).</p><p><b>METHODS</b>Eight genes fragements were amplified and ligated with bidirectional vector, recombinant plasmids were co transfected to the 293 T cells and rescued the virus. Gene sequencing, antigenic analysis and growth property were used to evaluate the rescued virus.</p><p><b>RESULTS</b>Rescued virus show the genes sequence correct, keep the same antigenicity and similar growth property compared with wild type virus.</p><p><b>CONCLUSION</b>The pandemic virus reverse genetics platform of A/California/07/2009 (H1N1) were built. Based on this platform, rescued virus hold the similarity of antigenicity and growth ability with wild type virus.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Orthomyxoviridae
/
Plasmides
/
Allergie et immunologie
/
Pandémies
/
Génétique inverse
/
Génétique
langue:
Zh
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2013
Type:
Article