Expression of adrenomedullin and its receptor mRNA in the tissues of normal adrenal medulla and pheochromocytoma / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 452-456, 2005.
Article
de Zh
| WPRIM
| ID: wpr-318886
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of human adrenomedullin (ADM) and its receptor-receptor activity modifying protein 2/calcitonin receptor-like receptor (RAMP2/CRLR) mRNA in the tissues of normal adrenal medulla and pheochromocytoma.</p><p><b>METHODS</b>Total RNA was extracted from normal adrenal medulla and pheochromocytomas. The expression of ADM and RAMP2/CRLR mRNA were studied by reverse transcription-polymerase chain reaction. The ratios of ADM/GAPDH, RAMP2/ GAPDH, CRLR/GAPDH were used to evaluate the expression levels of ADM, RAMP2 and CRLR mRNA.</p><p><b>RESULTS</b>Expressions of ADM and its receptor- RAMP2/CRLR mRNA were detected in normal adrenal medulla and pheochromocytoma tissues. ADM/GAPDH were 0.48+/-0.09 and 0.75+/-0.24, RAMP2/ GAPDH 0.79+/-0.12 and 1.29+/-0.30, CRLR/GAPDH 0.40+/-0.08 and 0.87+/-0.22 in normal adrenal medulla and pheochromocytomas, respectively (P < 0.05).</p><p><b>CONCLUSION</b>ADM exerts a possible autocrine or paracrine effect in the adrenal. ADM may be involved in the pathogenesis of pheochromocytoma.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Peptides
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Phéochromocytome
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ARN messager
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Peptide relié au gène de la calcitonine
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Récepteurs peptidiques
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Récepteurs à la calcitonine
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Tumeurs de la surrénale
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Médulla surrénale
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Protéines et peptides de signalisation intracellulaire
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Adrénomédulline
Limites du sujet:
Adult
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Female
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Humans
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Male
langue:
Zh
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2005
Type:
Article