Optimization of processing technology for xanthii fructus by UPLC fingerprint technique and contents of toxicity ingredient / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1248-1254, 2014.
Article
Dans Zh
| WPRIM
| ID: wpr-321329
Responsable en Bibliothèque :
WPRO
ABSTRACT
The experiment's aim was to optimize the processing technology of Xanthii Fructus which through comparing the difference of UPLC fingerprint and contents of toxicity ingredient in water extract of 16 batches of processed sample. The determination condition of UPLC chromatographic and contents of toxicity ingredient were as follows. UPLC chromatographic ACQUITY BEH C18 column (2.1 mm x 100 mm, 1.7 microm) eluted with the mobile phases of acetonitrile and 0.1% phosphoric acidwater in gradient mode, the flow rate was 0.25 mL x min(-1) and the detection wavelength was set at 327 nm. Contents of toxicity ingredient Agilent TC-C18 column (4.6 mm x 250 mm, 5 microm), mobile phase was methanol-0.01 mol x L(-1) sodium dihydrogen phosphate (35 65), flow rate was 1.0 mL x min(-1), and detection wavelength was 203 nm. The chromatographic fingerprints 16 batches of samples were analyzed in using the similarity evaluation system of chromatographic, fingerprint of traditional Chinese medicine, SPSS16.0 and SIMCA13.0 software, respectively. The similarity degrees of the 16 batches samples were more than 0.97, all the samples were classified into four categories, and the PCA showed that the peak area of chlorogenic acid, 3,5-dicaffeoylquinic acid and caffeic acid were significantly effect index in fingerprint of processed Xanthii Fructus sample. The outcome of determination showed that the toxicity ingredient contents of all samples reduced significantly after processing. This method can be used in optimizing the processing technology of Xanthii Fructus.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Acide quinique
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Acides caféiques
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Médicaments issus de plantes chinoises
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Chimie
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Chimie pharmaceutique
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Chromatographie en phase liquide à haute performance
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Classification
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Xanthium
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Toxicité
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Méthodes
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2014
Type:
Article