Effect of gambogic acid on proliferation of SKM-1 cells and its mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 105-109, 2013.
Article
Dans Zh
| WPRIM
| ID: wpr-325203
Responsable en Bibliothèque :
WPRO
ABSTRACT
The aim of this study was to explore the effect of gambogic acid (GA) on MDS SKM-1 cell proliferation, apoptosis and their possible mechanism. Cell proliferation was determined by MTT method. The apoptosis percentage and cell cycle regulation of SKM-1 cells were analyzed by flow cytometry. Morphological features were observed by light microscopy. The mRNA expression of bcl-2 and bax were detected by RT-PCR. The results showed that GA could inhibit the proliferation of SKM-1 cells in a dose- and time-dependent manner (IC50 was 0.37 µg/ml at 48 h), increase the apoptotic percentage of SKM-1 cells, and arrest cell cycle at the G0/G1. The expression of bax mRNA was up-regulated while that of bcl-2 mRNA was down-regulated in SKM-1 cells treated with GA for 48 h. It is concluded that GA can induce apoptosis, which may be related to its effect of arresting cells at phase of G0/G1 and down-regulating bcl-2/bax ratio.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
/
Pharmacologie
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Syndromes myélodysplasiques
/
Apoptose
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Protéines proto-oncogènes c-bcl-2
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Lignée cellulaire tumorale
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Xanthones
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Prolifération cellulaire
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Protéine Bax
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Points de contrôle du cycle cellulaire
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2013
Type:
Article