Clinical significance of rapid detecting bone marrow BCL2/IGH and BCL6/IGH fusion genes in patients with diffuse large B cell lymphoma by multiplex PCR / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1370-1373, 2012.
Article
de Zh
| WPRIM
| ID: wpr-325257
Bibliothèque responsable:
WPRO
ABSTRACT
Diffuse large B cell lymphoma (DLBCL) is the most common aggressive non-Hodgkin's lymphoma (NHL), characterized by great heterogeneity in clinical manifestations and molecular genetics. This study was aimed to explore the clinical significance of applying multiplex PCR to detect BCL2/IGH and BCL6/IGH fusion genes in DLBCL. Multiplex PCR was used to detect bone marrow samples from 80 cases of DLBCL. The results showed that 12 patients (15%) carried BCL2/IGH or BCL6/IGH fusion genes, BCL2/IGH was found in 6 patients (7.5%), and BCL6/IGH in another 6 patients (7.5%). The patients with different molecular markers displayed different clinical features and outcomes. It is concluded that multiple PCR is rapid and accurate method to identify gene abnormalities in DLBCL, but further studying a quantitative or semi-quantitative assay for the expression of fusion genes is needed.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
/
Moelle osseuse
/
Protéines de fusion oncogènes
/
Lymphome B diffus à grandes cellules
/
Chaines lourdes des immunoglobulines
/
Protéines proto-oncogènes c-bcl-2
/
Protéines proto-oncogènes c-bcl-6
/
Réaction de polymérisation en chaine multiplex
/
Génétique
Type d'étude:
Prognostic_studies
Limites du sujet:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2012
Type:
Article