A nested multiplex PCR assay for rapid subtyping the prevailing HIV-1 strains in Guangxi / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 482-484, 2009.
Article
de Zh
| WPRIM
| ID: wpr-325504
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a rapid nested multiplex PCR assay for subtyping HIV-1 CRF01_AE, CRF07_BC, CRF08_BC, B, and C strains prevailing in Guangxi.</p><p><b>METHOD</b>Subtype-specific primers were designed for these subtypes based on their gag sequences. The subtypes of HIV-1 samples from Guangxi were determined by nested multiplex PCR and DNA sequencing and phylogenetic analysis, respectively, and then the sensitivity and the specificity of nested multiplex PCR were calculated.</p><p><b>RESULTS</b>Nested multiplex PCR could correctly classify the 5 known-subtype samples, and were not reactive to all HIV-negative samples. Of the 72 HIV-positive samples, 66 were correctly identified as CRF01_AE, CRF07_BC, CRF08_BC, and B by this assay, giving a sensitivity of 91.7% (66/72), and a specificity of 100%.</p><p><b>CONCLUSION</b>This assay is a simple, fast, and cost-effective subtyping method for HIV-1 CRF01-AE, CRF07_BC, CRF08_BC, and B strains prevailing in Guangxi.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phylogenèse
/
Virologie
/
Données de séquences moléculaires
/
Infections à VIH
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Chine
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Réaction de polymérisation en chaîne
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VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
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Classification
/
Amorces ADN
/
Économie
Type d'étude:
Health_economic_evaluation
/
Prognostic_studies
Limites du sujet:
Humans
Pays comme sujet:
Asia
langue:
Zh
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2009
Type:
Article