Everted intestinal sac method for quick finding absorption ingredients of Wuzhuyu decoction / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1399-1404, 2010.
Article
Dans Zh
| WPRIM
| ID: wpr-328112
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for quick finding the absorption ingredients of Wuzhuyu decoction in order to select the index to control its quality.</p><p><b>METHOD</b>The absorption of three concentration of Wuzhuyu decotion was investigated with the in vitro-everted intestinal sac model. The intestinal bag fluid of jejunum and ileum were collected in different time and the eight ingredients, which were evodiamine (Ev), rutaecarpine (Ru), limonin (Li), ginsenoside-Rb1, -Rg1, -Re (Rb1, Rg1, Re), isorhamnetin-3-O-beta-D-glucosyl(6''-->1'")-alpha-L-rhamnoside (Irs)and 6-gingerol (6-Gi), were detected by HPLC as the represent constituents in samples.</p><p><b>RESULT</b>Eight ingredients except Ru in samples could be detected, but Ev could not be detected in high concentration samples. The ratios between absorption ingredients were different from in Wuzhuyu decotion.</p><p><b>CONCLUSION</b>The in vitro-everted intestinal sac canc absorb the ingredients of Wuzhuyu decotion selectivity. Compare with the ileum, the jejunum can provide the more absorption information and faster, the best test time is 60-90 min.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Médicaments issus de plantes chinoises
/
Pharmacocinétique
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Chimie
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Rat Wistar
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Liquide extracellulaire
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Évaluation préclinique de médicament
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Iléum
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Absorption intestinale
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Jéjunum
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Méthodes
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2010
Type:
Article