Fermentation of Bacillus subtilis ge25 strain and preliminary study on its antagonistic substances / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2624-2628, 2014.
Article
Dans Zh
| WPRIM
| ID: wpr-330281
Responsable en Bibliothèque :
WPRO
ABSTRACT
Panax ginseng is one of the most important traditional Chinese herbal medicine, soil borne diseases influenced the yield and quality severely. In our previous work, endophytic Bacillus subtilis ge25 strain was isolated from ginseng root, and which showed significant antagonistic activity against several most destructive ginseng phytopathogens. In the present work, crude protein and lipopeptid extracts were prepared from LB and Landy supernate by salting out, acid precipitation methods respectively. The antagonistic activity of crude extracts and stability to temperature and protease digestion were examined by ginseng phytopathogen Alternaria panax. Results showed that, the antagonistic activity of crude protein extracts from LB culture was complete and partially lost when treated by high temperature and proteinase K. However, crude lipopeptid from Landy culture showed significant stabile antagonistic activity to them. Acid-hydrolyzation and TLC-bioautography analysis showed, that the crude lipopeptide contained at least one cyclic lipopeptide. In consideration of the stability and perfect antagonistic activity of ge25, further researches will promote the biocontrol of ginseng diseases in the field.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
/
Physiologie
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Température
/
Bacillus subtilis
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Protéines bactériennes
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Racines de plante
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Endopeptidase K
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Alternaria
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Lipopeptides
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Fermentation
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2014
Type:
Article