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Isolation, culture and identification of neural stem cells in new-born rats / 华中科技大学学报(医学)(英德文版)
Article de En | WPRIM | ID: wpr-330867
Bibliothèque responsable: WPRO
ABSTRACT
The cortexes were obtained from new-born rats and dissociated to single cells by triturating. The cells were cultured in neural stem cell (NSC) culture medium (DMEM supplemented with bFGF, EGF and B27) and formed primary neurospheres after 7 days. Single cells dissociated from neurosphere were cultured in 96-well plates and formed single-cell cloning neurosphere 7 days later. The primary and single-cell cloning neurospheres were both positive for the immunofluorescent staining of nestin and were identified as NSC. It was proved that NSC can be expanded in vitro and provide seed cells for neural tissue engineering.
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Cellules souches / Séparation cellulaire / Cellules cultivées / Cortex cérébral / Rat Sprague-Dawley / Milieux de culture / Biologie cellulaire / Ingénierie tissulaire / Animaux nouveau-nés / Neurones Type d'étude: Diagnostic_studies Limites du sujet: Animals langue: En Texte intégral: J. huazhong univ. sci. tech. med. sci Année: 2004 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Cellules souches / Séparation cellulaire / Cellules cultivées / Cortex cérébral / Rat Sprague-Dawley / Milieux de culture / Biologie cellulaire / Ingénierie tissulaire / Animaux nouveau-nés / Neurones Type d'étude: Diagnostic_studies Limites du sujet: Animals langue: En Texte intégral: J. huazhong univ. sci. tech. med. sci Année: 2004 Type: Article