Changes of neuronal calcium channel following brain damage induced by injection of pertussis bacilli in rats / 中华创伤杂志(英文版)
Chin. j. traumatol
; Chin. j. traumatol;(6): 224-227, 2002.
Article
de En
| WPRIM
| ID: wpr-332963
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore changes of neuronal calcium channel following brain damage induced by injection of pertussis bacilli in rats, and to investigate the relationship between cytosolic free calcium concentration ([Ca(2+)](i)) in the synaptosome and Ca(2+)-ATPase activities of mitochondria.</p><p><b>METHODS</b>The level of [Ca(2+)](i) in the synaptosome and Ca(2+)-ATPase activities of mitochondria in the acute brain damage induced by injection of pertussis bacilli (PB) in rat was determined and nimodipine was administrated to show its effects on [Ca(2+)](i) in the synaptosome and on alteration of Ca(2+)-ATPase activity in the mitochondria. Seventy-three rats were randomly divided into four groups, ie, normal control group (Group A), sham-operation control group (Group B), PB group (Group C) and nimodipine treatment group (Group D).</p><p><b>RESULTS</b>The level of [Ca(2+)](i) was significantly increased in the PB-injected cerebral hemisphere in the Group C as compared with that in the Group A and the Group B at 30 minutes after injection of PB. The level of [Ca(2+)](i) was kept higher in the 4 hours and 24 hours subgroups after the injection in the Group C (P<0.05). In contrast, the Ca(2+)-ATPase activities were decreased remarkably among all of the subgroups in the Group C. Nimodipine, which was administered after injection of PB, could significantly decrease the [Ca(2+)](i) and increase the activity of Ca(2+)-ATPase (P<0.05).</p><p><b>CONCLUSIONS</b>The neuronal calcium channel is opened after injection of PB. There is a negative correlation between activities of Ca(2+)-ATPase and [Ca(2+)](i). Nimodipine can reduce brain damage through stimulating the activities of Ca(2+)-ATPase in the mitochondria, and decrease the level of [Ca(2+)](i) in the synaptosome. Treatment with nimodipine dramatically reduces the effects of brain damage induced by injection of PB.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Synaptosomes
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Bordetella pertussis
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Lésions encéphaliques
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Inhibiteurs des canaux calciques
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Nimodipine
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Calcium
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Analyse de variance
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Rat Sprague-Dawley
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Calcium-Transporting ATPases
Limites du sujet:
Animals
langue:
En
Texte intégral:
Chin. j. traumatol
Année:
2002
Type:
Article