Cloning, expression and purification of interferon-kappa, a novel human interferon, and its antiviral activity / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 223-226, 2005.
Article
Dans Zh
| WPRIM
| ID: wpr-333036
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To prepare human interferon-k (hIFN-kappa) and study its biological activities.</p><p><b>METHODS</b>Whole length of hIFN-kappa's cDNA was cloned, and its sequence was chemically synthesized according to the optimized codons of E.coli, then was expressed in E.coli DH5alpha. After purified, the rhIFN-kappa protein was tested for its various kinds of biological activities.</p><p><b>RESULTS</b>The purity of rhIFN-kappa was above 90%. In WHIS-VSV system, the antiviral activity of rhIFN-kappa was 2.0 x 10(6) IU/mg. Compared with rhIFN-alpha-2b, the biological activities of rhIFN-kappa were all feeble, including antiviral activity, promoting NK cell activity and anti-proliferation activity.</p><p><b>CONCLUSION</b>Antiviral activities of rhIFN-kappa on cell lines of different species are different, different viruses show different sensitivity to rhIFN-kappa.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Antiviraux
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Pharmacologie
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Plasmides
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Cellules Vero
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Protéines recombinantes
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Cellules tueuses naturelles
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Interféron de type I
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Tests de sensibilité microbienne
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Expression des gènes
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Lignée cellulaire
Limites du sujet:
Animals
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Humans
langue:
Zh
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2005
Type:
Article