Increased expression of serum gelsolin in patients with osteosarcoma / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 262-269, 2012.
Article
de En
| WPRIM
| ID: wpr-333504
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Identification of potential serum biomarkers of osteosarcoma to aid in its early diagnosis and in the discovery of possible therapeutic targets is an area of increasing interest.</p><p><b>METHODS</b>Two-dimensional difference-in-gel electrophoresis was used to assess multiple serum samples in patients with osteosarcoma. In addition, differential expression of protein biomarkers was characterized in osteosarcoma serum by using matrix-assisted desorption/ionization time-of-flight mass spectrometry coupled with database interrogation. Serum samples from four individuals with osteosarcoma and four age- and sex-matched healthy control subjects were compared.</p><p><b>RESULTS</b>Fifty-eight significant protein spot features in the osteosarcoma sera were found. These spot features were excised, digested with trypsin, and analyzed with mass spectrometry. Gelsolin was down-regulated only in osteosarcoma. Furthermore, Western blotting and enzyme linked immunosorbent assay (ELISA) confirmed decreased levels of gelsolin in the osteosarcoma serum samples.</p><p><b>CONCLUSIONS</b>These results indicated that gelsolin might have great potential as a biomarker of osteosarcoma and as a potential target for gene therapy.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sang
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Test ELISA
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Électrophorèse bidimensionnelle sur gel
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Marqueurs biologiques tumoraux
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Ostéosarcome
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Technique de Western
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Gelsoline
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Spectrométrie de masse MALDI
Type d'étude:
Screening_studies
Limites du sujet:
Adolescent
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Adult
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Child
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Female
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Humans
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Male
langue:
En
Texte intégral:
Chinese Medical Journal
Année:
2012
Type:
Article