CD147 and matrix metallo-proteinase (MMP) 2 and MMP9 expression in multidrug resistant breast cancer cells treated with P-glycoprotein substrate drugs / 中华病理学杂志
Chinese Journal of Pathology
; (12): 247-252, 2007.
Article
de Zh
| WPRIM
| ID: wpr-333915
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effects of P-glycoprotein (gp) substrate drugs on the expression of CD147 and MMP2 and 9 in multidrug resistant breast cancer cells.</p><p><b>METHODS</b>MDR human breast cancer cell line, MCF7/AdrR, and its sensitive parental line, MCF7, were treated with various concentrations of P-gp substrate drugs, including paclitoxel and vincristine, and P-gp nonsubstrate drugs, bleomycin, in serum-free media. At the end of the treatment, expressions of CD147 and MMP2 and 9 were determined by real-time PCR and western blot.</p><p><b>RESULTS</b>Increased expressions of CD147 and MMP2 and 9 were observed in multidrug resistant cancer cells compared with their parental MCF7 cells. After treatment with bleomycin, the expression of CD147 and MMP2 and 9 in both MCF7 and MCF7/AdrR cells remained unchanged (P > 0.05). However, treatment with paclitoxel and vincristine resulted in a remarkable over-expression of CD147 and MMP2 and 9 at both transcription and protein levels in MCF7/AdrR cell line (P < 0.05), while MCF7 cells failed to show similar response.</p><p><b>CONCLUSIONS</b>P-gp substrate drugs can greatly upregulate the expression of CD147 and MMP2 and 9 in multidrug resistant breast cancer cells, therefore enhancing the tumor metastatic capability.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Tumeurs du sein
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ARN messager
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Régulation de l'expression des gènes tumoraux
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Glycoprotéine P
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Multirésistance aux médicaments
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Résistance aux médicaments antinéoplasiques
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Matrix metalloproteinase 2
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Matrix metalloproteinase 9
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Lignée cellulaire tumorale
Limites du sujet:
Female
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Humans
langue:
Zh
Texte intégral:
Chinese Journal of Pathology
Année:
2007
Type:
Article