Tyrosine kinase JAK2V617F mutation in human myeloproliferative disorders / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 541-544, 2009.
Article
Dans Zh
| WPRIM
| ID: wpr-334073
Responsable en Bibliothèque :
WPRO
ABSTRACT
This study was aimed to investigate the JAK2V617F mutation in myeloproliferative disorders (MPD) and to evaluate the significance of JAK2V617F in diagnosis and therapy of MPD. The bcr/abl fusion gene in 70 MPD patients was detected by reverse transcription polymerase chain reaction (PT-PCR). The JAK2V617F mutation was detected by allele-specific polymerase chain reaction (AS-PCR) and the results were confirmed by sequence analysis. The results indicated that the bcr/abl fusion gene could be detected in 38 patients with chronic myeloid leukemia (CML), but not in the 32 none-CML patients. The JAK2V617F mutation was detected in 12 out of 16 (75%) patients with polycythemia vera (PV), 3 out of 10 (30%) patients with essential thrombocythemia (ET), 3 out of 6 (50%) patients with idiopathic myelofibrosis (IMF), but not in any of the CML patients. The JAK2V617F mutation frequencies between CML and bcr/abl negative MPD patients were statistically significant (p < 0.05). It is concluded that the JAK2V617F may be a characteristic molecular event in PV, ET and IMF patients which may serve as an important molecular marker for the diagnosis and classification of the three diseases.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Réaction de polymérisation en chaîne
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Mutation ponctuelle
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Kinase Janus-2
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Génétique
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Méthodes
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Syndromes myéloprolifératifs
Limites du sujet:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2009
Type:
Article