Preparation and identification of the monoclonal antibody specific to hemagglutinin of avian paramyxovirus type 2 / 病毒学报
Chinese Journal of Virology
; (6): 148-151, 2008.
Article
Dans Zh
| WPRIM
| ID: wpr-334833
Responsable en Bibliothèque :
WPRO
ABSTRACT
A hybridoma cell line 1G4A7 secreting monoclonal antibody (McAb) specific to hemagglutinin of avian paramyxovirus type 2 (APMV-2) was developed by fusing the spleen cells of APMV-2 immunized BAlb/c mice with SP2/0 myeloma cells. The immunoglobulin subclass of 1G4A7 was IgG1 with light chain kappa and the affinity constant against APMV-2 was 1.02 X 10(10). Identified by HI and indirect ELISA, the McAb titers in ascities were 10 log 2 and 1 10(6) respectively. The McAb did not cross react with the common avian viruses, showing good specificity. There existed obvious differences in antigenitic relationship among APMV-2 viruses analyzed by HI and indirect ELISA using McAb 1G4A7.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Test ELISA
/
Tests d'inhibition de l'hémagglutination
/
Avulavirus
/
Allergie et immunologie
/
Hémagglutinines virales
/
Souris de lignée BALB C
/
Anticorps monoclonaux
Type d'étude:
Diagnostic_studies
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Virology
Année:
2008
Type:
Article