Antitumor effect of GPI-CD80 fusion protein in nude mice / 南方医科大学学报
Journal of Southern Medical University
; (12): 1027-1029, 2007.
Article
de Zh
| WPRIM
| ID: wpr-337331
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the antitumor effect GPI-CD80 fusion protein and its mechanisms.</p><p><b>METHODS</b>A tumor vaccine was prepared by culturing HepG2 cells in the presence of purified GPI-CD80 followed by inactivation with mitomycin, with mitomycin-inactivated HepG2 cells as the control group. The two preparations were co-cultured with nude mouse splenic lymphocytes, and the changes of lymphocyte proliferation and the production of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) were detected by MTT assay. The cytotoxic T-lymphocyte (CTL) activity was evaluated by LDH-release assay, and the changes of gross tumor volume were measured in tumor-bearing nude mice after administration of different vaccines.</p><p><b>RESULTS</b>The application of GPI-CD80 tumor vaccine resulted in significantly increased optical density, IL-2 and IFN-gamma levels and CTL activity of the nude mouse splenic lymphocytes in comparison with the control groups. The average tumor volume in nude mice treated with GPI-CD80 tumor vaccine was significantly smaller than that in negative control and blank control groups.</p><p><b>CONCLUSION</b>GPI-CD80 fusion protein may inhibit the tumor growth velocity in nude mice, possibly by promoting lymphocyte proliferation, stimulating the production of the cytokines IL-2 and IFN-gamma, and enhancing of CTL activity.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Rate
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Protéines de fusion recombinantes
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Cricetulus
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Interféron gamma
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Interleukine-2
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Cellules CHO
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Glycosylphosphatidylinositols
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Antigène CD80
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Vaccins anticancéreux
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Biologie cellulaire
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2007
Type:
Article