Inhibitory effects of kappa-opioid receptor stimulation on cultured myocardial cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 358-361, 2002.
Article
de Zh
| WPRIM
| ID: wpr-339714
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>AIM</b>In order to study the effects of kappa-opioid receptor activation, we used cultured cardiomyocytes to study the inhibitory effects of U50,488H on cellular proliferation, protein content in the presence or absence of nor-binaltorphimine (nor-BNI).</p><p><b>METHODS</b>The cellular proliferation was determined with crystal violet staining and the protein content was assayed with Lowry's method.</p><p><b>RESULTS</b>A kappa-opioid receptor agonist U50,488H at 0.1 micromol/L-10 micromol/L inhibited the cellular proliferation and protein content of cultured myocardial cells in a dose-dependent manner. The inhibitory effects of U50,488H were completely blocked by pretreatment with nor-BNI, a specific kappa-opioid receptor antagonist at 1 micromol/L.</p><p><b>CONCLUSION</b>U50,488H inhibited the cultured myocardial cells' growth. The inhibitory effects of U50,488H are involved in mediating the action of kappa-opioid receptor stimulation.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Cellules cultivées
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Rat Sprague-Dawley
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Récepteur kappa
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2-(3,4-Dichlorophényl)-N-méthyl-N-((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)acétamide
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Myocytes cardiaques
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Processus de croissance cellulaire
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Métabolisme
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2002
Type:
Article