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Expression and molecular evolution of recombinant acetylcholinesterase for detection of pesticide residues: a review / 生物工程学报
Chinese Journal of Biotechnology ; (12): 557-564, 2012.
Article de Zh | WPRIM | ID: wpr-342461
Bibliothèque responsable: WPRO
ABSTRACT
Acetylcholinesterase (AChE) plays a key role in the pesticide determination. However, the extraction of AChE from natural materials has the disadvantages of low yield, complex purification and poor stability. Therefore, the preparation of recombinant AChE with high performance becomes the hot topic of researchers in recent years. In this article we summarize the progress in the expression of recombinant AChE and the improvement of its analytical characteristic. Finally, we point out that the directed evolution strategy combined with surface display technology is the future trend on improving recombinant AChE activity.
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Pichia / Acetylcholinesterase / Protéines recombinantes / Résidus de pesticides / Chimie / Anticholinestérasiques / Baculoviridae / Évolution moléculaire / Techniques d'exposition à la surface cellulaire / Vecteurs génétiques Type d'étude: Diagnostic_studies langue: Zh Texte intégral: Chinese Journal of Biotechnology Année: 2012 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Pichia / Acetylcholinesterase / Protéines recombinantes / Résidus de pesticides / Chimie / Anticholinestérasiques / Baculoviridae / Évolution moléculaire / Techniques d'exposition à la surface cellulaire / Vecteurs génétiques Type d'étude: Diagnostic_studies langue: Zh Texte intégral: Chinese Journal of Biotechnology Année: 2012 Type: Article