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High-level production of alkaline polygalacturonate lyase in recombinant Pichia pastoris / 生物工程学报
Chinese Journal of Biotechnology ; (12): 635-639, 2008.
Article de Zh | WPRIM | ID: wpr-342858
Bibliothèque responsable: WPRO
ABSTRACT
In order to increase the production of alkaline polygalacturonate lyase (PGL) by recombinant Pichia pastoris GS115, the effect of cell and methanol concentration on the PGL production was carefully investigated by single factor experiment. The optimum conditions were listed as follows: the cell concentration 122 g/L, the methanol concentration 20 g/L, and the ratio of methanol and cell concentration 0.16-0.20 g/g (methanol/cell). With the glycerol and methanol feeding strategies, the ratio of methanol and cell concentration could be controlled at the range of 0.171 to 0.195 g/g. And the highest PGL activity (430 u/mL) and highest PGL productivity (4.34 u/mL/h) were achieved.
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Pichia / Polysaccharide-lyases / Recombinaison génétique / Protéines recombinantes / Chimie / Méthanol / Alcalis / Fermentation / Vecteurs génétiques / Génétique langue: Zh Texte intégral: Chinese Journal of Biotechnology Année: 2008 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Pichia / Polysaccharide-lyases / Recombinaison génétique / Protéines recombinantes / Chimie / Méthanol / Alcalis / Fermentation / Vecteurs génétiques / Génétique langue: Zh Texte intégral: Chinese Journal of Biotechnology Année: 2008 Type: Article