Fluorescence in situ hybridization on bone marrow smear in the detection of cytogenetic aberrations of multiple myeloma / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1230-1233, 2009.
Article
de Zh
| WPRIM
| ID: wpr-343313
Bibliothèque responsable:
WPRO
ABSTRACT
This study was aimed to establish the technique of interphase fluorescence in situ hybridization (I-FISH) used on smear of bone marrow directly, and to develop a new method for detection of the molecular cytogenetics in multiple myeloma (MM). After a series of treatment, fixation and digestion of the bone marrow smear as the carrier, the chromosome 8 centromere probe were used in I-FISH for molecular cytogenetics detection. At the same time, differences were compared in the results between the new method and the conventional I-FISH. The results showed that there was no statistically significant difference of proportion of various signals in non-hematologic malignancies when detected with the two methods (p>0.05). In bone marrow smear I-FISH, 8 out of 19 cases (42.1%) had abnormality of chromosome 8, including 5 cases with -8 (26.3%) and 3 cases with +8 (15.8%). It is concluded that the I-FISH on smear of bone marrow is characterized by convenience, economy and accuracy. Therefore, it can be used for research of molecular cytogenetics in MM.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
/
Moelle osseuse
/
Chromosomes humains de la paire 8
/
Aberrations des chromosomes
/
Hybridation fluorescente in situ
/
Génétique
/
Méthodes
/
Myélome multiple
Type d'étude:
Diagnostic_studies
Limites du sujet:
Adult
/
Aged
/
Female
/
Humans
/
Male
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2009
Type:
Article