Establishment and application of a method for assessing hemopoietic chimerism in rhesus after allogeneic stem cell transplantation / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 683-686, 2005.
Article
Dans Zh
| WPRIM
| ID: wpr-343905
Responsable en Bibliothèque :
WPRO
ABSTRACT
Monitoring engraftment of donor cells after allogeneic transplantation is the key of assessing successful establishment of animal transplantation model. The purpose of this study was to establish a method for analysis of chimerism in rhesus transplantation model. Y-specific sequence in rhesus was amplified by the polymerase chain reaction (PCR), method for analysis of chimerism in rhesus after sex-mismatched transplantation was established; the feasibility and sensitivity of the approach were tested by using serial DNA mixtures of sex-mismatched individuals; the accuracy of results was confirmed by chromosome karyotype analysis simultaneously; Chimerisms of one rhesus received allogeneic stem cell transplantation and the other received mesenchymal stem cells (MSC) transfusion were detected by this method. The results showed that a 176 bp long sequence of PCR product was gained in male rhesus, while no product was gained in female rhesus. The sensitivity of this method was up to 0.05% (male/female DNA ratio). Male donor chimerism were found on day 7 and 14 after allogeneic stem cell transplantation by Y-specific sequence and chromosome karyotype analysis. Otherwise, male donor chimerism was found in peripheral blood at 1 hour and in bone marrow on day 30 after MSC transfusion by this method, but no male donor chimerism was found after MSC transfusion using chromosome karyotype analysis. In conclusion, this rapid, sensitive approach can used to assess chimerism in experiments of rhesus alloorgan transplantation and cell transfusion.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Transplantation homologue
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Chromosome Y
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Sang
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Données de séquences moléculaires
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Séquence nucléotidique
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Chimère obtenue par transplantation
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Modèles animaux
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Transplantation de cellules souches mésenchymateuses
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Génétique
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Macaca mulatta
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2005
Type:
Article