Analysis of RASSF1A promoter hypermethylation in serum DNA of non-small cell lung cancer / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 284-287, 2008.
Article
de Zh
| WPRIM
| ID: wpr-348112
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the hypermethylation status of RASSF1A promoter in serum DNA of non-small cell lung cancer (NSCLC) patient and evaluate its correlation with clinicopathological parameters.</p><p><b>METHODS</b>Serum DNA was extracted from the peripheral blood of 75 NSCLC patients and another 35 patients with benign pulmonary disease and 15 healthy donors. The methylation status of RASSF1A promoter was determined using methylation-specific PCR (MSP), and the correlation of methylation profiles with clinicopathological parameters was statistically analyzed.</p><p><b>RESULTS</b>Aberrant methylation of RASSF1A was detected in 23 of 75 (30.7%) cancer patients, but in none of patients with benign pulmonary disease or in healthy donors (P <0.001). RASSF1A hypermethylation status was found to be correlated with late stage and poor differentiation (P < 0.05), but not with gender, age or histopathology in NSCLC patients.</p><p><b>CONCLUSION</b>Hypermethylated RASSF1A promoter is frequently found in the serum DNA of non-small cell lung cancer patient, and RASSF1A may become a promising novel biomarker for diagnosis and prognosis prediction in lung cancer.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Sang
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ADN tumoral
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Marqueurs biologiques tumoraux
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Études cas-témoins
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Réaction de polymérisation en chaîne
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Régions promotrices (génétique)
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Carcinome pulmonaire non à petites cellules
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Méthylation de l'ADN
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Protéines suppresseurs de tumeurs
Type d'étude:
Observational_studies
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Risk_factors_studies
Limites du sujet:
Adult
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Aged
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Female
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Humans
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Male
langue:
Zh
Texte intégral:
Chinese Journal of Oncology
Année:
2008
Type:
Article