Peptides analysis in digested edible bird's nest by HPLC-MS / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 714-719, 2013.
Article
Dans Zh
| WPRIM
| ID: wpr-350699
Responsable en Bibliothèque :
WPRO
ABSTRACT
Edible bird's nest contains lots of glycoproteins. The glycosylation inhomogeneity for glycoprotein often results in wide range of molecular weight and the difficulty for protein separation and charaterization. In this paper, proteins in the edible bird's nest were extracted using multiple extractions, and then digested by PNgase F and trypsin. The digest mixture was separated with HPLC, and peptides were identified based on MS/MS data searching. The results indicated that the extracted proteins were amount to 79.7% of total protein in the edible bird's nest. More than 20 species of peptides in the digested mixture were identified. The sequences of these peptides showed similarity with some proteins from Swiss-prot. The research indicated that deglycosylation, tryptic digestion coupled with HPLC-MS/MS is a proper strategy for characterization of proteins in the edible bird's nest.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Fragments peptidiques
/
Spectrométrie de masse
/
Oiseaux
/
Glycoprotéines
/
Chimie
/
Chromatographie en phase liquide à haute performance
/
Séquence d'acides aminés
/
Protéolyse
/
Médecine traditionnelle chinoise
/
Métabolisme
Type d'étude:
Diagnostic_studies
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2013
Type:
Article