Effects of compound Salvia injection on number and activity of endothelial progenitor cells / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 246-249, 2006.
Article
de Zh
| WPRIM
| ID: wpr-350962
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Compound Salvia injection (CSI) on the number and activity of endothelial progenitor cells (EPCs).</p><p><b>METHOD</b>Mononuclear fraction of human umbilical cord blood was obtained by density gradient centrifugation and plated on fibronectin coated culture dishes. Cells were divided in to five groups: group control, group VEGF, group CSI 50, group CSI 10 and group CSI 2 (supplemented with none cytokine, VEGF 10 ng x mL(-1), CSI 50, 10, 2 microg x mL(-1), respectively). After six days in culture, cell clusters were viewed with an inverted microscope, fluorescence-activated cell sorting (FACS) analysis of PE-CD34 and FITC-VE-Cadherin was performed to detect number of EPCs, adhesion assay was performed by replating cells on fibronectin coated dishes, and then counting adherent cells.</p><p><b>RESULT</b>Numbers of EPCs of group VEGF, group CSI 10 and group CSI 2 were significantly increased as compared with those of group control ( P < 0.01, P < 0.05, P < 0.01, respectively), and numbers of EPCs of group CSI 2 were more than those of group CSI 10 and group V (P < 0.01). Compared with group control, number of EPCs of group CSI 50 was significantly decreased (P < 0.01). Compared with group control, numbers of clusters and adhesive EPCs of group CSI 10 and group CSI 2 were significantly increased, while those of group CSI 50 were significantly decreased.</p><p><b>CONCLUSION</b>Low concentration CSI can significantly promote EPCs augmentation and enhance its functional activity, while high concentration CSI significantly restrains it.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Plantes médicinales
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Cellules souches
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Hémogramme
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Médicaments issus de plantes chinoises
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Endothélium vasculaire
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Adhérence cellulaire
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Chimie
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Salvia miltiorrhiza
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Biologie cellulaire
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Zhongguo Zhong Yao Za Zhi
Année:
2006
Type:
Article