Application of Combined Detection of Fusion Gene and BIOMED-2 Standardized Ig Gene Rearrangement System in Childhood B-cell Acute Lymphoblastic Leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 352-357, 2016.
Article
de Zh
| WPRIM
| ID: wpr-360086
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the application of combined detection of fusion gene and BIOMED-2 standardized immunoglobulin (Ig) gene rearrangement system in diagnosis and treatment of children with acute lymphoblastic leukemia (ALL).</p><p><b>METHODS</b>Multiplex-PCR amplifications and RQ-PCR of RNA/DNA were performed using ALL fusion gene detection kit and BIOMED-2 primer. The Ig gene rearrangements were analyzed by using PCR fragment analysis system.</p><p><b>RESULTS</b>Out of 251 children with B-ALL, 77 cases were TEL-AML1(+) , 28 cases were E2A-PBX1(+) , 10 cases were MLL-AF4(+) , 11 cases were BCR-ABL(+) , the total positive rate was 50.2%, 82.5% showed IgH VH-JH rearrangement, 53.4% showed IgK rearrangement. The positive rate of combined detection of fusion gene and gene rearrangement was 99%. E2A-PBX1(+) and MLL-AF4(+) with IgK(+) gene rearrangement group was compared with negative control group, the difference was statistically significant (P < 0.001 or P = 0.005); 105 ALL fusion gene positive cases had been detected by fluorescence in situ hybridization (FISH) simultaneously, the accordance rate of fusion gene and FISH was more than 94%.</p><p><b>CONCLUSION</b>The combined detection of ALL fusion gene and BIOMED-2 standardized clonality analysis system can improve the positive detected rate of B-ALL dramatically, and make the grouping of disease prognosis more accurately; this combined detection is a more faster and sensitive method than FISH.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protéines de fusion oncogènes
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Protéines de fusion bcr-abl
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Hybridation fluorescente in situ
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Amorces ADN
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Diagnostic
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Sous-unité alpha 2 du facteur CBF
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Leucémie-lymphome lymphoblastique à précurseurs B et T
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Réaction de polymérisation en chaine multiplex
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Recombinaison V(D)J
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Génétique
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites du sujet:
Child
/
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2016
Type:
Article