Effects of xeroderma pigmentosum B gene on proliferation and apoptosis of vascular smooth muscle cells induced by interleukin-6 / 中华老年医学杂志
Chinese Journal of Geriatrics
; (12): 849-853, 2011.
Article
Dans Zh
| WPRIM
| ID: wpr-422600
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WPRO
ABSTRACT
Objective To investigate effects of xeroderma pigmentosum B(XPB) gene on IL-6 induced proliferation and apoptosis in human vascular smooth muscle cells (VSMC).Methods Recombinant plasmid pcDNA3.1-XPB and vacant vector plasmid pcDNA3.1 were transfected stably into VSMC by liposome,and these cells were incubated with IL-6 at a 100 U/ml for 48 hours.The experiments were divided into six groupsblank control group; pcDNA3.1 group; pcDNA3.1-XPB group;IL-6 group; IL-6 + pcDNA3.1 group; IL-6 + pcDNA3.1-XPB group.The expression levels of XPB,Bcl-2,Bax and wild type p53 (wt-p53) were detected through reverse transcription polymerase chain reaction (RT-PCR) and Western blotting.The cell survival,cell cycle and apoptosis were examined with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry,respectively.Results The transfection of pcDNA3.1-XPB increased the expression of XPB,Bax and wt-p53 (P<0.05 or P<0.01),decreased the expression of Bcl-2 (P<0.05 or P<0.01),and reduced the IL-6 induced effects on decreasing the expression of Bax and wt-p53 and increasing the expression of Bcl-2(P<0.05 or P<0.01).The over expression of XPB inhibited the cell growth(q=2.95,P< 0.05),and reduced the positive effects of IL-6 on VSMC growth ( 102.6 +6.2) % vs.(124.5 + 7.9) %,q=3.49,P<0.05.The over expression of XPB increased the apoptosis rate of VSMC(P<0.01 ) and the cell amounts of G0/G1 phase (q=2.99,P< 0.05),decreased the cell amounts of S phase(q=3.05,P<0.05),and reduced the IL-6 induced effects on decreasing the apoptosis rate of VSMC(5.9±2.1)% vs.(0.3±0.1)%,q=7.53,P<0.01; the cell amounts of G0/G1 phase(70.9±6.7) % vs.(54.8±2.9) %,q=6.91,P<0.01 ;and on increasing the cell amounts of Sphase(20.2+3.6)% vs.(36.4+7.2)%,q=8.54,P<0.01.Conclusions XPB gene could inhibit VSMC proliferation,promote VSMC apoptosis,and reduce the effects that IL-6 promotes VSMC proliferation and inhibits VSMC apoptosis.Therefore,XPB gene is likely to be potential molecular target for treatment of atherosclerosis.
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WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Geriatrics
Année:
2011
Type:
Article